The July 1986 Nairobi conference of the Circle of Concerned African Women Theologians commissioned four groups to examine the patriarchal structures that determined African women’s lives in their religious, cultural, economic and social settings. The Religion and Culture Commission, which was the fourth commission, translated into the ‘Institute of Women in Religion and Culture (IWRC)’ in 1999 at Trinity Theological Seminary, Legon Ghana. The IWRC set out as its agenda to organize public education on gender sensitivity and gender justice in religion and culture as it affects women’s daily lives in Ghana. The public education targeted women, faithbased organization and traditional leaders seeking a biblical, Qur’anic and cultural hermeneutics that recognize and promote the humanity of women. The liberative theology that emanated from this approach sought to transform women’s lives in their families, churches, academic and social settings. This chapter aims at highlighting the developments of the Institute from its inception in 1999 to 2022. It explores the developments of the Institute, achievements and challenges to see how the Institute has been relevant to their well-being in the areas of religion and culture.